Isolation, Identification, and Bioefficacy of Indigenous Entomopathogenic Fungi Against Pentalonia nigronervosa Coq. in Abaca

Abstract

Abaca (Musa textilis Nee) remains at risk due to Banana bunchy top virus and Abaca bunchy top virus, vectored by Pentalonia nigronervosa Coq. Present management practices primarily rely on chemical insecticides, raising environmental and sustainability concerns. This study explores the biocontrol potential of indigenous entomopathogenic fungi (EPF) sourced from aphids and rhizospheric soil in abaca-growing areas. Four fast-growing and sporulating ones were selected from twenty fungal isolates for molecular identification via the internal transcribed spacer (ITS) region. AEPF5 was 100 % similar to Fusarium sp., AEPF9 as Penicillium sp. (97 %), SEPF1 as Talaromyces funiculosus (100 %), and SEPF5 as Trichoderma sp. (99 %). SEPF5 and AEPF9 were the most aggressive and effective, with the highest mortality rates of 70.00 % and 64.44 % after 12 hrs, respectively. All isolates were comparable to the used commercial insecticide with 100 % mortality after 48 hrs. Trichoderma sp. showed rapid infectivity and robust pathogenicity. Penicillium sp. exhibited potent bioactivity but was marginally slower. Conversely, Fusarium sp. displayed limited pathogenicity, and Talaromyces sp. showed varied pathogenicity. Results affirm the promise of indigenous EPF as effective, reducing reliance on synthetic chemicals, improving ecological balance, and strengthening the resilience of abaca farming communities. This approach contributes to regenerative pest management strategies that align with sustainable fiber-based agroecosystems.