Molecular Detection of Cellular Prion Protein in Brain Tissues of Black Bengal Goats in Bangladesh

Abstract

The cellular prion protein (PrPC) is a membrane-bound glycoprotein mainly
present in the central nervous system which is necessary for the establishment
and further evolution of prion disease in human and animals. The aim of the
present study was to investigate the PrPC protein in brain tissues of black Bengal
goat. Fifteen brain tissues were collected from different slaughter houses of
three districts (Mymensingh, Manikgonj and Netrokona) of Bangladesh during
January to February, 2011. The PrPC protein was detected in the brain tissues
of black Bengal goats using polymerase chain reaction. The result showed all
positive (100%) of the amplified samples. The standardized PCR could be used
for detection of PrPC protein in different tissues of animals and humans.
Sequencing of PrP gene in the black Bengal goats for the risk assessment of
scrapie is needed for further study. To our knowledge, detection of PrPC protein
in the brain tissues of indigenous goats is the first time in Bangladesh.